Yeast carboxypeptidase Y can be translocated and glycosylated without its amino-terminal signal sequence. Genetic analysis of protein export in Escherichia coli K12. Expression of genes in yeast using the ADCI promoter. Nucleotide sequence of the triose phosphate isomerase gene of Saccharomyces cerevisiae. Links to PubMed are also available for Selected References. Get a printable copy (PDF file) of the complete article (1.8M), or click on a page image below to browse page by page. Full textįull text is available as a scanned copy of the original print version. Two of three mutants tested produced lower steady-state levels of alpha-factor in intact yeast cells, although the magnitude of the effect was less than that in the cell-free system. After reassembly into an intact alpha-factor gene, the substitutions were found to decrease up to 50-fold the rate of translocation of prepro-alpha-factor across microsomal membranes in vitro. The mutations resulted in replacement of a residue in the hydrophobic core of the signal sequence with either a hydrophilic amino acid or a proline. Signal sequence mutants were obtained by selecting for cells that partially mistargeted into mitochondria a fusion protein consisting of the alpha-factor signal sequence fused to the mature portion of an imported mitochondrial protein (Cox IV). We used a genetic approach to identify point mutations in the signal sequence of a secreted eucaryotic protein, yeast alpha-factor.
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In momentum or trend-following strategies, you’re just buying assets that had been past winners and, selling assets that had been past losers.(在动量或者趋势策略中,你只是买入过去的赢家的资产,卖出去亏损的资产) This is the opposite of a mean reversion strategy where you sell winners and buyback losers. In contrast to correlation when you test for co-integration, use prices rather than returns since you’re more interested in the trend between the variable’s means over time than in the individual price movements. Often variables with high correlation will also be co-integrated and vice versa, but this isn’t always the case. Co-integration tasks do not measure how well two variables move together, but rather whether the difference between their means remains constant. These two assets are correlated, but the difference between their means is not stable. Notice how the returns in this example track closely, and then diverge by about 10 percent, and then by about 15 percent. The change in prices is then the cumulative return on each asset(每种资产的累计收益). When you look for a correlation in a price series, you normalize the prices so that each starts at 100 percent. Positive correlation means that the variables move in tandem in the same direction while negative correlation means that they move in tandem but in opposite directions. Correlation coefficients(相关性系数) range from negative one or perfect negative correlation to zero, or no correlation to one which is perfect positive correlation. Correlation is a measure of how well two variables move together over time. When the price risk spread gets too low, it becomes more likely that it will increase, and again revert to its mean. When the price risk spread gets too high, it becomes more likely that it will decrease, and so revert to its long-term mean or neutral value. Mean reversion strategies(均值回归策略) depend on the stability or stationarity of an asset’s price or spread relative to another asset(依赖于一项资产的价格或相对于另一项资产的价差的稳定性或者平稳性). These forecasts are based on an observed price response to factors that you have identified as statistically significant in predicting changes in an instrument’s price. Quant strategies are also a type of forecasting method that attempts to predict the future value of a stock or other instrument where the direction of a price spread between two instruments. You must implement high-frequency trading strategies with software and market interfaces that can handle sub-millisecond order creation and submission. Strategies range from low-frequency, at least by trading standards, to high-frequency. You can use this analysis to uncover hidden patterns and behaviors in the market for particular asset or group of assets such as an index(指数). Rules can be quite complex and are a product of systematic statistical analysis of an asset’s historical price data(对资产历史价格数据系统数据分析的产物). Quant strategies are a precise set of rules that generate orders and manage the risk of your current position(量化交易是一套精准的规则,用于生成订单和管理当前头寸风险). In this section, we will explore the design and implementation of quant trading strategies. The level of tactics in Desperados III and Shadow Tactics are even higher than Hard West imho. There is a demo to try Thank you very much! Desperados is a very old series and it's pretty cool someone made a new game! I'd check it out. Originally posted by I can reccomend You the third game. Some weapons might be simply more powerful and easier to use, while other weapons might be weaker and require a plan to be useful. Story heavily relies on communication skills and looting skills if you want to have a say. Wasteland 2: Innovative CLASSIC attribute system for you to build different types of combatants. Default attributes could be "developed" by innovative mechanism of wounds, scars, and talent card combos. Loot and conversation requires crazy save scumming if you care about it. Inventory of gears, quest items, and a lot of junks. Our decisions (skills required) change how the story evolves and how NPC interact with you. A main quest line, with side quests and random encounters. Hard West: Short stories interwound but not interdependent. A rough strategic direction change should suffice to alter the result for most fights. Wasteland 2: Well built character skills and superior firepower are more decisive. Overall, the fights are not too difficult after you know well how it works. On the other hand, if you could get all the advantages before each combat and accumulate all of them before the final combat, you could also become overly powerful. If you enjoy surprises and don't mind the added challenges by entering the combat with disadvantages, I think you won't complain. However, they are very random, not following the same mechanism each time. There is a preparation phase before each combat, which decides your beginning stats for the fight. In the hardest cases (usually the first or the last fight), I had to try several times to figure out how to solve the ambush for example. One slight error may lead to a complete fail. The information at this website might include opinions or views which, unless expressly stated otherwise, are not necessarily those of the 911METALLURGIST.COM or 911METALLURGY CORP or any associated company or any person in relation to whom they would have any liability or responsibility.Hard West: Well thought out strategic movement is crucial, down to the tactical level. Visitors to the 911METALLURGIST.COM website should not act upon the website’s content or information without first seeking appropriate professional advice.ĩ11METALLURGY CORP accepts no responsibility for and excludes all liability in connection with browsing this website, use of information or downloading any materials from it, including but not limited to any liability for errors, inaccuracies, omissions, or misleading statements. The 911METALLURGIST.COM and 911METALLURGY CORP tries to provide content that is true and accurate as of the date of writing however, we give no assurance or warranty regarding the accuracy, timeliness, or applicability of any of the contents. Perhaps exclude all results either result is not at least 5x detection limit.ĭISCLAIMER: Material presented on the 911METALLURGIST.COM FORUMS is intended for information purposes only and does not constitute advice. Previous comment about including detection limit data is a good one. I'm not saying that this is a good value but you need to have a think about how critical this is for your ore-waste decisions. Suggest if you are getting 90% of results with a hard difference of less than 40%, then that would be pretty typical of a lot of gold deposits around the world. Also check what proportion of the charge is actually rock - if you have a lot of sulphide you may have an issue there. Next think to check is for coarse gold - have you done any screen fire assaying? If you have some coarse gold you may need to increase the charge of your fire assay to get better repeat precision. The first thing to check is how is the duplicate pulp collected - if is taken from jus the top of the bag this is a problem. Unless your gold is very fine then you are likely to get a fair bit of noise from the pulp duplicates. 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The English-language Quora website is a thriving bank of knowledge that attracts large investors and prominent supports – e.g. The direct exchange of knowledge can be fun, thanks to the successful feedback mechanism of the platform. The potential, though, is certainly there: after all, the platform is easy to use and they would be able to achieve a lot with a large, capable community. International reach is not strong enough and there are not enough users worldwide to turn this into a reality. The goal of creating a comprehensive database of user content that can compete with Wikipedia seems like a particularly lofty goal. However, those who are just getting started on Quora are likely to be confronted with a jumble of trivial, often confused questions. In principle, Quora needs a continuous feed of users that are experts or educated people with different areas of expertise to comment on a range of current questions. This results in Quora having a fast, dynamic user experience. If you want to contribute, you can do so without having to leave the feed. The feed is a mix of active threads, unanswered questions that match your selected categories, and suggestions on how users can complete their profile. Quora’s homepage feed is reminiscent of Facebook’s timeline and allows visitors to use the site seamlessly with unlimited scrolling. As a concept, Quora lies somewhere between a social network, like Facebook, and a knowledge forum like Mind the Book, or Yahoo! Answers. 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Not only that, but up and coming producer YPOnTheBeat has been tearing the streets up lately, becoming one of the most in-demand producers in the Bay Area. Having first seen success at the age of 14 with a spot on BET’s 106 and Park, Young Bari continues to go strong-and all without ever cursing on record. Paying homage to Cash Money’s Big Tymers, Nef goes in on a minimal beat to let you know: Bitch he’s big tymin'! Now, with “Big Tymin',” he has another clap on his hands. Ooooooh, I’m ballin like Baby! Twenty-year-old Vallejo MC Nef The Pharaoh has seen his buzz shoot through the roof lately, with a local hit last year in “Bitch I’m From Vallejo,” a feature on E-40’s Sharp On All 4 Corners album, an appearance in Thizzler's Bay Area Freshmen Ten Class of 2014. It’s a very heartfelt song over a somber flip of Aerosmith's “Dream On.” But with The Jacka’s passing, A-Wax expresses his regret over how things went down and even laments that if things had gone differently he would have been honored to have been the sixth Mob Figa. Sbvce)įor as long as I can remember, A-Wax has had a legendary beef with the Mob Figaz, even going so far as to brawl in the street with Jacka and Husalah at SXSW a few years back. Here he snaps on a sentimental sampled beat with his homie Dam Theuz, who also blazes the track.Ī-Wax - “Rest In Peace To The Jacka” (prod. At 17 years old, he’s crafted a signature sound, with production similar to Curren$y or early Wiz. RAZO is a youngster we’ve been watching for the last year and a half or so, and he continues to impress us. Now he hopes to continue that trend with “Hit Or Nah,” a West Coast club anthem that now features New York’s French Montana. Rayven Justice had a decent sized hit last year with “Slide Thru,” which dominated the FM airwaves. HD and G-Dirty profess their love to their girls, but they aren’t talking about your average ladies they love white girl (cocaine), their ninas (nine millimeter pistols), and chasing Goapele (guap a.k.a. But unlike many street artists, HD delivers more gems than duds, and this track, released on Valentine’s Day, is one of them. In keeping with the Oakland independent style, HD favors quantity over quality, putting out tape after tape and single after single. G-Dirty - “Girl Krazy”Īn Oakland staple, HD got out of jail a few years back and has been dropping heat ever since. With the new bilingual cut “AyAyAy!,” Snow goes in over a bass heavy Blue The Misfit production, switching effortlessly between English and Spanish and topping it off with a hypnotic hook for the Latinas. San Jose to Texas’s Snow Tha Product is RAW! We’ve been watching Snow for a minute now, and we're pretty confident she can gas any competition, regardless of gender. The Bay's eyes really are all on him, and he makes it look easy to deliver with this introspective joint produced by the slap master DJ Mustard. HBK Gang’s iamsu! keeps killing it: He recently announced his solo Eyes On Me tour, organized and performed at a packed HBK Day event, and dropped his new Eyes On Me EP. On “Killa,” Alier mixes fast paced lyricism with a smooth melodic hook, waxing philosophical on his past relationships. Fast forward to now, and this is the first single off the -presented album High N’ Love. The homie Chris Mixx played through his whole upcoming LP, and I was blown away. I (Matt) discovered Alier Johnson, who was born in Chicago and raised in Oakland, a month or two back after a studio session with Thizzler affiliate Magic City Studios. On this standout joint off What Happened To The World, The Jacka links with partners in mob Dru Down and Joe Blow for a somber, echo-filled ode to dope money.Īlier Johnson - “Killa” (prod. The Jacka was on a great run over the past year, releasing a solo mixtape ( What Happened To The World) and the long awaited Highway Robbery album with Freeway, so it’s even sadder that he was gunned down with so much more to come on the horizon. The Bay took a huge blow to the heart last month with the loss of the legendary Mob Figaz member The Jacka in a late night Oakland shooting. Dru Down and Joe Blow - “The President’s Face” Volume Two of On The Roof, dedicated to The Jacka, is below, with descriptions of each track in the mix: At Noisey, we love Bay Area rap, so we've teamed up with our friends over at Thizzler on the Roof to present a mix of some of the best the region has to offer right now. Still, music new and old continues to exist, and heartfelt tributes to and signs of the The Jacka's influence continue to emerge. To re-do your pointer movements or slide timings, just repeat these steps. Download 25 Best Mouse Cursors or Pointers for Windows 11/10 Watch on You can use these cursors on Windows 11, 10, 8.1, and 7. To view the pointer movements and timings you just recorded, on the Slide Show tab, in the Start Slide Show group, click either From Beginning or From Current Slide. Specifications U-Pointer Technology Image Processing Technology Refresh Rate 60 fps Response Time 16.7 ms Auto Calibration Operating Distance Follow Projector Specifications Multi-User Multiple Operation 2 (Dual) Supported OS Windows: WinXP, Vista, 7, 8, 8.1 Troubleshooting 1. The recorded slide show timings are automatically saved and the slide show appears in Slide Sorter view with timings under each slide. To end your slide show recording, right-click the slide, and then click End Show. To move to the next slide, release Ctrl, and then click the left mouse button. Press and hold Ctrl, and then click and drag the left mouse button to point to the contents on the slide that you want to draw attention to. Tip: To pause the narration, in the Recording box, click Pause. In the Record Slide Show dialog box, select the Narrations and laser pointer check box. Ĭlick either Start Recording from Beginning or Start Recording from Current Slide, depending on where you want to start to record your slide show. Here, you can download some cool mouse cursors or pointers to replace your boring default Windows Mouse Pointers/cursors. Any pointer in this regard will be highly appreciated. Download Mouse Cursors for Windows for free. But if not, Ill be willing to use any minimalist-esque third-party pointer if they look simple like the Windows Default pointer. On the Slide Show tab, in the Set Up group, point to the arrow next to Record Slide Show. I like the simple Windows Default pointer in Windows 10 so Id prefer if I can simply increase the size of it, if possible. To record your laser pointer movements to enhance a web-based or self-running slide show, do the following: While it is possible to make cursors of non-standard size, Windows will automatically resample them decreasing image quality.(For complete details about recording and timing your slide shows, see Record a slide show with narration and slide timings.) Important: always create 32x32 pixels cursors unless you have a serious reason not to. If you have found that someone has used your pictures and added them to the library without your permission, contact us immediately at and they will be removed. If you are using pictures downloaded from internet to create cursors, you must make sure the author of the pictures Important: only cursor authors or copyright holders may add cursor to the library. Multiple cursors with similar theme and you would like to have them turned into a cursor set, register or login and return to this page. The cursors are by default added to the "Junkyard".This helps people to find your cursor when using the search box. Carefully choose file name and fill in the description.When uploading, please use the same email address associated with your account on this server.Open a cursor file, and click the command "Cursor"->"Add to Online Library" from application menu.Get RealWorld Cursor Editor (it's free).If you are an author of a cursor or cursor set, you may present your work to visitors Here you can change the cursors used by Windows in different If you do not want to install any software, just click on Control Panel in Start menu, then double-click the Mouse icon and Beside changing cursors one by one, the tool also allows exporting and importing cursor schemes. If you change your cursors frequently, consider using a free tool that allows you toĬhange cursor from Windows Explorer context menu. Despite the name, there are some worthy pieces. Such submittions end up on the cursor junkyard. Not everybody uploads enough cursors to create a cursor sets. By Evan.L 349 hi use them Browse all cursor setsĬlick on a number above to display page with 40 cursor sets. Wells, Winston Churchill, Nikola Tesla, Harry Houdini, and even Arthur Conan Doyle himself. On the other hand, the show is also known for its knack of authenticity, with constant reference and appearances of stalwart Victorian figures such as H.G. fingerprints, blood testing, and even sonar (some of these technologies explained as prototypes of the actual breakthroughs). Set in late-nineteenth-century Canada, the show features the protagonist William Murdoch, a detective in the Toronto constabulary, solving cases using methods that are far ahead of their time, and are often on the brink of anachronistic forensic devices – i.e. The Artful Detective Plot: What is it about? Additionally, the entire lead crew returns to reprise their original roles. Season 13 sees a guest appearance by Colm Feore as well as reunites Constable Crabtree with his father – George Crabtree Sr. The series has lived up to a most notorious reputation for historical figures and guest stars – having cast cosmetics industry juggernaut and famous entrepreneur Florence Nightingale Graham (founder of Elizabeth Arden, Inc.) as a nursing student with whom Crabtree has a brief liaison, with a wink here and a nod there as a quite opaque reference to her real-life cosmetics empire. Comedian Colin Mochrie, who has also guest-starred in the series in 2017, jocularly commented that he was the only actor left in Canada to not have previously played a guest role in the show. The show’s usual ensemble cast returns to their respective roles, the phenomenal Yannick Bisson as the main lead William Murdoch, Helene Joy as the doctor and coroner Julia Ogden, Thomas Craig as Brackenreid, as well as Jonny Harris as Crabtree.Īdditionally, the show is also known for its surprise stunt castings – having made shockers such as William Shatner as Mark Twain, and most famously, the former Canadian Prime Minister Stephen Harper. Now the question is, will there be a ‘The Artful Detective’ season 13? We will delve into that question, but first, let’s quickly look into the details of the series. The series then went on to be renewed by Citytv for four consecutive seasons.Īfter its season 5 premiere, the platform changed, and this time the show was picked up by CBC who acts as its distributor. The long-running show has had 12 successful seasons so far. The first season was received with widespread acclaim and it went on to receive 14 Gemini Awards nominations. In its first season, Outerbridge was replaced by Yannick Bisson to play the titular character. It started airing in its current format from 2008 on Citytv in Canada. The series was originally released as a two-episode TV movie back in 2004 with Peter Outerbridge starring as William Murdoch. The characters appearing on the show are all inspired by the novels. The source materials for the series’ adaptation are the Detective Murdoch novels written by Maureen Jennings. First launched in 2008, the show is a combination of a Sherlock-Esque eccentricity, real history, and the classic detective show formula. Carney, Cal Coons, and Alexandra Zarowny. It is the best that has happened to Ovation since its inception. Murdoch Mysteries (also known as The Artful Detective in the US where it is broadcasted on the Ovation cable TV network) is a crime drama series developed by R.B. Instead, I opted for choosing the upward direction and was happy that some health increases were peppered in between larger costing upgrades.Ī two-weapon setup, air dash, deflection shield, oh and you can eat your enemies. I didn’t take the time to map out the way I wanted to go based on the furthest points because it was a lot of reading. There are a ton of upgrades in the massive webbed bracket. A skill tree can be found afterwards to improve your character. Collect as much fat and protein from eating and attempt a few other missions then vomit it out at the end of the level. Bonus levels and areas are found in the Mega Man X capsules and will have mini-games or wagers to be made with shady characters. Anything can be considered food as this day and age allows it with the help of technology. New weapons can be found in levels and dropped by baddies and can be upgraded when a certain “food” is eaten. Enjoy some references to Mega Man, Pokémon, and Major League Eaters throughout. Pick your player and hop into this decaying world chock full of run and gun action, while eating anything you find along the way.Īs a hired mercenary, the mission is to gorge yourself with vegetables, drones, and zombies alike to collect genetic material. This game was developed by Mega Cat Studios, maybe you’re familiar with Coffee Crisis, or Phantom Fear? All made by the talented retro heads at Mega Cat Studios. Ghouls now contaminate the Earth and two mercenaries are hired by DarwinCorp to wreak havoc and collect genetic material. Technology was used to overcome such issues but ultimately failed when it caused mutations. Bite The Bullet sets the stage with its story possibly reflecting parts of our world in economic collapse and starvation. Here you will find options to view and activate subscriptions, manage institutional settings and access options, access usage statistics, and more.Binge eating, forced vomiting, competitive eaters, and cursing asparagus.Īll of these things are what make Bite The Bullet different from the rest of the games on the eShop. If you believe you should have access to that content, please contact your librarian.įor librarians and administrators, your personal account also provides access to institutional account management. The institutional subscription may not cover the content that you are trying to access. Oxford Academic is home to a wide variety of products. 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